time-resolved fluorescence
- time-resolved fluorescence
-
Method to avoid interference by autofluorescence. Using an emitter fluorochrome that has slow decay characteristics coupled to the reagent of interest and temporally separating excitation and measurement, the signal can be arranged to derive almost entirely from the reporter fluorophore. (Autofluorescence decays very rapidly.) See
HTRF.
Dictionary of molecular biology.
2004.
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